| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1521 | Thermosipho melanesiensis | GGCGATGGTGGAAGCGTTA | GACCACCTTCACCTGCAGT | 60.45 | 59.55 | 244 | 33 |
100.00%
|
100.00%
|
| Primer 1522 | Thermosipho melanesiensis | ATCGAGTCCCCAGGTGGAA | TCGCAACCATAGGAAGCCT | 59.92 | 58.70 | 235 | 33 |
100.00%
|
100.00%
|
| Primer 1523 | Thermosipho melanesiensis | AATCGAGTCCCCAGGTGGA | TCGCAACCATAGGAAGCCT | 59.92 | 58.70 | 236 | 33 |
100.00%
|
100.00%
|
| Primer 1524 | Thermosipho melanesiensis | ACCTTCCACCTGGATACGATG | CCCCTTCTCCCCTGCTTTA | 59.24 | 58.29 | 245 | 33 |
100.00%
|
100.00%
|
| Primer 1525 | Thermosipho melanesiensis | CCTTCCACCTGGATACGATGA | CCCCTTCTCCCCTGCTTTA | 58.96 | 58.29 | 244 | 33 |
100.00%
|
100.00%
|
| Primer 1526 | Thermosipho melanesiensis | CCCCAACAAGGTAGCAGGT | CGGCATACAAGTCTTTCCCCT | 59.23 | 60.07 | 155 | 33 |
100.00%
|
100.00%
|
| Primer 1527 | Thermosipho melanesiensis | TGCAGACGCGAAGGAATGA | TTGCGCTTCAAGAACTCCAT | 59.71 | 58.10 | 186 | 32 |
100.00%
|
100.00%
|
| Primer 1528 | Thermosipho melanesiensis | ACCTGCAACTATCGGAGCA | TCCAACGCCTGCTTGTATGA | 58.71 | 59.68 | 226 | 32 |
100.00%
|
100.00%
|
| Primer 1529 | Thermosipho melanesiensis | CAGCACGTGGTGGAGTTGT | TATGCGGAGTTGCGACGA | 60.52 | 59.12 | 159 | 31 |
100.00%
|
100.00%
|
| Primer 1530 | Thermosipho melanesiensis | TCTCTTCAATGGGAACTGGGT | ACGCGATCCTTGCTATAACGA | 58.65 | 59.66 | 176 | 31 |
100.00%
|
100.00%
|